Primary Information |
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| BoMiProt ID | Bomi10713 |
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| Protein Name | Zinc finger protein 148/Transcription factor ZBP-89/Zinc finger DNA-binding protein 89 |
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| Organism | Bos taurus |
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| Uniprot ID | Q3Y4E1 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001028293.1 |
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| Aminoacid Length | 794 |
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| Molecular Weight | 89003 |
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| FASTA Sequence |
Download |
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| Gene Name | ZNF148/ZBP89 |
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| Gene ID | 613265 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | transcriptional regulation,negative role in human muscle cell differentiation. |
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| Biochemical Properties | contains Zinc finger region.C2H2-type 1,C2H2-type 2,C2H2-type 3,C2H2-type 4 involved in DNA binding and metal binding. |
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| PTMs | Acetylation, Isopeptide bond formation, Phosphorylation, Ubl conjugation,Sumoylation on K356 |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q3Y4E1|ZN148_BOVIN Zinc finger protein 148 OS=Bos taurus OX=9913 GN=ZNF148 PE=2 SV=1
MNIDDKLEGLFLKCGGIDEMQSSRAMVVMGGVSGQSTVSGELQESVLQDRS*51MPHQEILAA
DEVLQESEMRQQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITF
TDVSEQLMRDKKQIREPVDLQKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAF
RTNYHLQRHVFIHT*194GEKPFQCSQCDMRFIQKYLLQRHEKIHTGEKPFRCDECGMRFIQKY
HMERHKRTHS*250GEKPYQCEYCLQYFSRTDRVLKHKRMCHENHDKKLNRCAIKGGLLTSEED
S*301GFSTS*306PKDNSLPKKKRQKTEKKSSGMDKESSLDKSDLKKDKNDYLPLYSSSTKVKDEYM
VAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTIS*412PLSTYEES
KVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAA
SNNSREYALNVGTIASQPSVTQAAVASVIDESTTASILDSQALNVEIKSNHDKNVIPDEV
LQTLLDHYSHKANGQHEISFSVADTEVTSSISINSSEVPEVTQSENVGSSSQASSSDKAN
MLQEYSKFLQQALDRTSQNDAYLNSPSLNFVTDNQTLPNQPAFSSIDKQVYATMPINSFR
SGMNS*665PLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASATSTQDFLDQVTSQKKAEAQPV
HQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFPEFPLVNVNDNRAG
MTSS*784PDATTGQTFG
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| Predicted Disorder Regions | 49-67, 570-622, 716-734, 781-794 |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Significance of PTMs | Phosphorylated by ERK at Ser306 and this results in ZNF148 binding to FOX1 which furthur facilitates Histone H3 acetylation and FOXM1-mediated Snail transcription and promotes cell invasion and tumor growth.Sumoylated by SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes. |
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| Additional Comments | Downregulation of ZNF148 drives the formation of a muscle phenotype with rapid expression of myosin heavy chain, even in proliferative conditions. |
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| Bibliography | 1.Gao X, Ma C, Sun X, Zhao Q, Fang Y, Jiang Y, Shen K, Shen X. Upregulation of ZNF148 in SDHB-deficient gastrointestinal stromal tumor potentiates Forkhead box M1-mediated transcription and promotes tumor cell invasion. Cancer Sci. 2020 Apr;111(4):1266-1278. doi: 10.1111/cas.14348. Epub 2020 Mar 16. PMID: 32060966; PMCID: PMC7156819. |
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