Primary Information |
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| BoMiProt ID | Bomi5373 |
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| Protein Name | E3 ubiquitin-protein ligase RNF128/RING finger protein 128/RING-type E3 ubiquitin transferase RNF128 |
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| Organism | Bos taurus |
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| Uniprot ID | Q29RU0 |
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| Milk Fraction | Exosomes |
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| Ref Sequence ID | NP_001069539.1 |
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| Aminoacid Length | 431 |
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| Molecular Weight | 46881 |
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| FASTA Sequence |
Download |
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| Gene Name | RNF128 |
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| Gene ID | 535869 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Protein Function | RNF128 as an E3 ligase for K63-linked ubiquitination and activation of TBK1 and delineated a previously unrecognized function for RNF128. |
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| Biochemical Properties | RNF128 directly interacted with TBK1 through its protease-associated (PA) domain and catalyzed the K63-linked polyubiquitination of TBK1, which led to TBK1 activation, IRF3 activation and IFN-β production. |
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| PTMs | N-Linked Glycosylation at Asn, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q29RU0|RN128_BOVIN E3 ubiquitin-protein ligase RNF128 OS=Bos taurus OX=9913 GN=RNF128 PE=2 SV=1
MGQLPGAGVFCRGGCGFSRLLAWCFLLVLSPQTPGSRGAEAVWTAYLN*48VSWRVPHTGVN*59R
TVWELSEEGVYGQDSPLEPVAGVLVPPDGPGALNACNPHTN*101FTVPTVPGDWGSSVQVSWL
ALIQRGGGCTFADKIHLAYERGASGAVIFNFPGTRNEVIPMSHPGAGDIVAIMIGNLKGT
KILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFIITAATVGYFIFYSARRLRNA
RAQSRKQRQLKADAKKAIGRLQLRTQKQGDKEIGPDGDSCAVCIELYKPNDLVRILTCNH
VFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNETSSNASPHEEDN
RSETASSGYASVQGADEPPLEEHAHSANENLQLVNHEANSMAVDVVPHVDNPTFEEDESP
DQETTVREIKS |
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| Predicted Disorder Regions | 245-269, 337-431 |
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| DisProt Annotation | |
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| TM Helix Prediction | 1TMH; (209-231) |
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| Additional Comments | Deficiency of RNF128 expression attenuated IRF3 activation, IFN-β production and innate antiviral immune responses to RNA and DNA viruses, in vitro and in vivo. |
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| Bibliography | 1.Song G, Liu B, Li Z, Wu H, Wang P, Zhao K, Jiang G, Zhang L, Gao C. E3 ubiquitin ligase RNF128 promotes innate antiviral immunity through K63-linked ubiquitination of TBK1. Nat Immunol. 2016 Dec;17(12):1342-1351. doi: 10.1038/ni.3588. Epub 2016 Oct 24. PMID: 27776110. 2. |
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