Primary Information |
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| BoMiProt ID | Bomi7109 |
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| Protein Name | Microprocessor complex subunit DGCR8/DiGeorge syndrome critical region 8 homolog |
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| Organism | Bos taurus |
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| Uniprot ID | A6QR44 |
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| Milk Fraction | whey |
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| Ref Sequence ID | NP_001094674.1 |
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| Aminoacid Length | 760 |
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| Molecular Weight | 84383 |
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| FASTA Sequence |
Download |
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| Gene Name | DGCR8 |
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| Gene ID | 540254 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Protein Function | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Involved in the silencing of embryonic stem cell self-renewal |
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| Biochemical Properties | DGCR8 possesses MAPK docking motifs that match both of the recently structurally defined motifs that are specific for JNK and ERK/p38 kinases.All 23 DGCR8 phosphosites in the N terminus,is required for nuclear localization and for the ability of DGCR8 to homodimerize. |
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| Significance in milk | involved with biological processes in response to heat stress effects |
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| PTMs | Phosphorylation,Ubl conjugation,isopeptide bond formation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A6QR44|DGCR8_BOVIN Microprocessor complex subunit DGCR8 OS=Bos taurus OX=9913 GN=DGCR8 PE=2 SV=1
METCGSPSPLPREPAGGVAMEDRARPLRALPRGQS*35PPPPLQTSSDAEVMDVGSGGDGQAE
PPAEDPLNFYGASLLSKGSSSKARLLVDPNCS*92GHS*95PRTARHAPAVRKFSPDLKLLKDVKI
SVSFTESCRSEDRKVLYTGAERDVRAECGLALSPVIGDVHAGPFGGSVGNGVGAGGESAG
KRDEEHELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFSYE
DDFDNDVDALLEEGLCAPKKRRMEEKYGGDS*271DHPS*275DGET*279SVQPMMTKIKTVLKSRGRPPT
EPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHGPPLTSIPCLHYRKMKD
SEERERAAGIAPPEPELPPDEPDPLGTDAGPPDEKDPLGAEAAPGALGQVKAKVEVCKDE
SVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLS
VQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYSFFECENPSEPFGASVTIDGVTY
GSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPRDSEELEYFNHISIEDSRVYELTS
KAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGK
QLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQFARKNKPNLHILS
KLQEEMRRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
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| Predicted Disorder Regions | 19-53, 77-85, 169-202, 378-396, 747-749 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | DGCR8 possesses MAPK docking motifs that match both of the recently structurally defined motifs that are specific for JNK and ERK/p38 kinases.DGCR8 can be phosphorylated by mitogenic ERK/MAPK, indicating that DGCR8 phosphorylation may respond to and integrate extracellular cues. |
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| Additional Comments | DGCR8 function is clearly important, as it is essential for viability in mice and DGCR8-knockout embryonic stem cells show a proliferation defect.DGCR8 deficiency in the brain has also been suggested to cause behavioral and neuronal defects associated with the 22q11.2 deletion syndrome known as DiGeorge syndrome. |
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| Bibliography | 1.Herbert, K. M., Pimienta, G., DeGregorio, S. J., Alexandrov, A., & Steitz, J. A. (2013). Phosphorylation of DGCR8 increases its intracellular stability and induces a progrowth miRNA profile. Cell reports, 5(4), 1070–1081. https://doi.org/10.1016/j.celrep.2013.10.017 2.Landthaler, M., Yalcin, A., & Tuschl, T. (2004). The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Current biology : CB, 14(23), 2162–2167. https://doi.org/10.1016/j.cub.2004.11.001 |
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