Primary Information |
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| BoMiProt ID | Bomi8899 |
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| Protein Name | RNA-splicing ligase RtcB homolog/3'-phosphate/5'-hydroxy nucleic acid ligase |
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| Organism | Bos taurus |
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| Uniprot ID | Q5E9T9 |
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| Milk Fraction | Exosomes |
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| Ref Sequence ID | NP_001015631.1 |
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| Aminoacid Length | 505 |
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| Molecular Weight | 55199 |
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| FASTA Sequence |
Download |
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| Gene Name | RTCB |
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| Gene ID | 525106 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | spermatocyte |
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| Protein Function | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. |
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| PTMs | Isopeptide bond formation, Phosphorylation at Ser, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q5E9T9|RTCB_BOVIN RNA-splicing ligase RtcB homolog OS=Bos taurus OX=9913 GN=RTCB PE=2 SV=1
MSRSYNDELQFLEKISKNCWRIKKGFVPNMQVEGVFYVNDSLEKLMFEELRNACRGGGVG
GFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDI
NCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSL
REGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNE
YAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIAS*300
PEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQ
HVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMT
ETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTD
VVNTCHDAGISKKAIKLRPIAVIKG
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Knock down of RTCB was sufficient to restore sensitivity to tamoxifen in endocrine-resistant cells and increased the formation of aggresomes, leading to apoptotic cell death. |
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| Bibliography | 1.Direito I, Monteiro L, Melo T, Figueira D, Lobo J, Enes V, Moura G, Henrique R, Santos MAS, Jerónimo C, Amado F, Fardilha M, Helguero LA. Protein Aggregation Patterns Inform about Breast Cancer Response to Antiestrogens and Reveal the RNA Ligase RTCB as Mediator of Acquired Tamoxifen Resistance. Cancers (Basel). 2021 Jun 26;13(13):3195. doi: 10.3390/cancers13133195. PMID: 34206811; PMCID: PMC8269126. |
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