Primary Information |
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| BoMiProt ID | Bomi9051 |
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| Protein Name | Serine/threonine-protein kinase PRP4 homolog/PRP4 pre-mRNA-processing factor 4 homolog |
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| Organism | Bos taurus |
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| Uniprot ID | Q08DZ2 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001069025.1 |
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| Aminoacid Length | 1008 |
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| Molecular Weight | 116646 |
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| FASTA Sequence |
Download |
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| Gene Name | PRPF4B |
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| Gene ID | 512236 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | PRP4 kinase domain is responsible for promoting drug resistance to curcumin by inducing EMT.This protein kinase as a novel protein kinase required for regulation of proper chromosome segregation during meiosis |
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| PTMs | N6-Acetylation at Lys, Isopeptide bond formation, Phosphorylation at Ser/Thr, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q08DZ2|PRP4B_BOVIN Serine/threonine-protein kinase PRP4 homolog OS=Bos taurus OX=9913 GN=PRPF4B PE=2 SV=1
MAAAEAPS*8LREQPEMEDANS*20EKS*23VNEENGEVS*32EDQSQNKHSRHKKKKHKHRSKHKKHKHS
SEEDKDRKHKHKHKHKKHKRKEVADAS*87DKEGMS*93PAKRTKLDDLALLEDLEKQRALIKAEL
DNELMEGKVQS*131GMGLILQGY*140ES*142GS*144EEEGEIHEKARNGNRSSTRSSS*166TKGKLELVDNKNSTKKRSKSRSKERTRHRSDKKKSKGGVEIVKEKATRSKSKERKKSKSPSKRSKSQDEARKS*239K
S*241PTLRRRSQEKVGKARS*257PVDDKAKVEDKSKAKDRKKS*277PVINES*283RSRDRGKKS*292RS*294PVDLRGKSKDRRSRSKERKSKRPEADKEKKPVKS*328PSKDASSGKENRSPSRRPGRSPKRRS*354LS*356PKQRDKSRRS*366RS*368PLVNDRRSKQSKSPSRT*385LS*387PGRRAKSRSLERKRREPERRRLSSPRTRPRDDILSRRERS*427KDAS*431PISRWS*437PARRRASRSPVRRRSRSPLRRSRSPRRRSRSPRRRDRGRRSRSRLRRRSRSRGGRRRRSRSKVKEDKFKGSLSEGMKVEQES*519S*520S*521DDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLADDSNLSVPS*566EPSS*570PQSSTRS*577RS*579PS*581PDDILERVAADVKEYERENVDTFEASVKAKHNLMAVEQNNGSSQKKLLAPDMFTESDDMFAAYFDSARLRAAGIGKDFK
ENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKII
RNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLK
KYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSAS
HVADNDITPY*850LVS*853RFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLK
LAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADL
IGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQEKI |
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| Predicted Disorder Regions | 8-97, 133-567 |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Additional Comments | PRP4 overexpression altered the morphology, rearranged the actin cytoskeleton, triggered epithelial-mesenchymal transition (EMT), and decreased the invasiveness of HCT116 cells. |
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| Bibliography | 1.Ahmed MB, Islam SU, Sonn JK, Lee YS. PRP4 Kinase Domain Loss Nullifies Drug Resistance and Epithelial-Mesenchymal Transition in Human Colorectal Carcinoma Cells. Mol Cells. 2020 Jul 31;43(7):662-670. doi: 10.14348/molcells.2020.2263. PMID: 32576716; PMCID: PMC7398799. 2.Islam SU, Ahmed MB, Lee SJ, Shehzad A, Sonn JK, Kwon OS, Lee YS. PRP4 kinase induces actin rearrangement and epithelial-mesenchymal transition through modulation of the actin-binding protein cofilin. Exp Cell Res. 2018 Aug 1;369(1):158-165. doi: 10.1016/j.yexcr.2018.05.018. Epub 2018 May 19. PMID: 29787735. |
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